Calibration of HPLC and their Operation SOP
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Calibration of HPLC and their Operation SOP
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A Calibration curve based on n=10 known points was used to measure the protein in an unknown. The results were protein = 15.22 /- 0.46 micrograms, where the standard deviation is σ = 0.46 micrograms. Find the 90% and 99% confidence intervals for protein in the unknown. The book gives the answer of 15.22 /- 0.86 for the 90% CI and 15.22 /- 1.5 for the 99% CI.
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