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When DNA replicates, the enzyme helicase separates the DNA strands. Single-stranded DNA binding proteins then stabilize the unwound template DNA, keeping it in an extended single-stranded state so that it can be copied by DNA polymerase. One new DNA strand is called the leading strand, which forms from its 5′ to its 3′ end by the action of DNA polymerase III. A sliding-clamp forms a ring around the DNA and maintains the association of the DNA and the polymerase, allowing the uninterrupted synthe
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When DNA replicates, the enzyme helicase separates the DNA strands. Single-stranded DNA binding proteins then stabilize the unwound template DNA, keeping it in an extended single-stranded state so that it can be copied by DNA polymerase. One new DNA strand is called the leading strand, which forms from its 5′ to its 3′ end by the action of DNA polymerase III. A sliding-clamp forms a ring around the DNA and maintains the association of the DNA and the polymerase, allowing the uninterrupted synthe
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The replisome complex in action: During DNA replication, helicase unzips the parent strands while single stranded binding proteins stabilize each strand. Topoisomerase relieves tension by cutting and rejoining DNA downstream of the replication fork. The primase enzyme catalyzes an RNA primer so that DNA polymerase III (guided by a sliding clamp) can begin polymerizing the new DNA molecule from the template. On the leading strand, this happens continuously as helicase continues unzipping the pare
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